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KMID : 1101320070390030223
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Korean Journal of Clinical Laboratory Science
2007 Volume.39 No. 3 p.223 ~ p.230
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Comparative Analysis of Histochemical Stains about Detection of H. pylori in Gastric Mucosa
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Cha Min-Seon
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Abstract
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Several methods have been used in the detection of Helicobacter pylori (H. pylori) which was believed to be a pathogenic organism causing chronic gastritis, benign peptic ulcer, gastric carcinoma or malignant lymphoma. Even though several methods were introduced for detection of H. pylori in stomach, there were controversies in their sensitivities and specificities. This experiment were designed to study the comparative analysis of staining methods (hematoxylin and eosin (H&E), Giemsa, Warthin-Starry and immunohistochemical stain) to dectect H. pylori in the gastric mucosa. The results were as follows. Average density score of H. pylori classified by Genta were 2.29 in Warthin-Starry stain, 2.19 in Giemsa stain, 1.34 in immunohistochemical stain and 0.98 in H&E stain. By comparison between inflammatory degree by Sydney system and result of Warthin-Starry stain, the detection rate and densities of H. pylori were increased from mild (61.5% and 0.8), moderate (90.4 and 2.1), and severe (100% and 3.2). From the above findings, Warthin-Starry stain is useful method for detection of H. pylori in gastric mucosa.
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KEYWORD
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Gastric mucosa, Helicobacter pylori, Hematoxylin and eosin stain, Giemsa stain, Warthin-Starry stain, Immnnohistochemical stain
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